Click image to see more details
Product Info Summary
| SKU: | M06893-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Canine, Chicken, Human, Mouse, Rat, Swine |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TFIIB GTF2B Monoclonal Antibody
SKU/Catalog Number
M06893-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TFIIB GTF2B Monoclonal Antibody catalog # M06893-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Canine, Chicken, Human, Mouse, Rat, Swine.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFIIB GTF2B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06893-1)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
Mix
Isotype
IgG
Immunogen
Purified recombinant human TFIIB protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06893-1 is reactive to GTF2B in Bovine, Canine, Chicken, Human, Mouse, Rat, Swine
Applications
M06893-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.833kDa
Background of TFIIB
Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:500-1:1000
IF 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Immunofluorescent staining data of GTF2B using Anti-TFIIB GTF2B Monoclonal Antibody (M06893-1).
Immunofluorescence (IF) analysis of HeLa cells using TFIIB Monoclonal Antibody.
Click image to see more details
Figure 2. Immunohistochemistry validation of GTF2B using Anti-TFIIB GTF2B Monoclonal Antibody (M06893-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon using TFIIB Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-TFIIB GTF2B Monoclonal Antibody M06893-1
Western Blot (WB) analysis using TFIIB Monoclonal Antibody against JurkatProtein Target Info & Infographic
Gene/Protein Information For GTF2B (Source: Uniprot.org, NCBI)
Gene Name
GTF2B
Full Name
Transcription initiation factor IIB
Weight
34.833kDa
Superfamily
TFIIB family
Alternative Names
general transcription factor IIB; General transcription factor TFIIB; S300-II; TFIIBTF2BRNA polymerase II transcription factor IIB; transcription initiation factor IIB GTF2B TF2B, TFIIB general transcription factor IIB transcription initiation factor IIB|RNA polymerase II transcription factor IIB|S300-II|general transcription factor TFIIB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GTF2B, check out the GTF2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GTF2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TFIIB GTF2B Monoclonal Antibody (M06893-1)
Hello CJ!
No publications found for M06893-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TFIIB GTF2B Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-TFIIB GTF2B Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
